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Boris Johnson has urged people to seize the chance to break away from "a failing EU system" on Wednesday.
On a final trip of the Leave campaign trail, Mr Johnson visited London, Ashby de la Zouch, Selby and Darlington to try and persuade undecided voters that Britain would be best leaving the EU.
ITV News Political Correspondent Libby Wiener reports:
David Cameron toured the UK alongside former Prime Ministers Gordon Brown and John Major on Wednesday, on a final day of campaigning for Britain to Remain in the EU.
Mr Cameron said he thought his campaign was "very strong", but when questioned on immigration, he added "you don't tackle immigration by tanking our economy".
ITV News Deputy Political Editor Chris Ship reports:
Nigel Farage will miss the final televised EU Referendum debate on Wednesday evening.
Mr Farage was due to appear on Europe: The Final Debate with Jeremy Paxman on Channel 4 from 9pm - the day before the polls open.
A spokesman for the Ukip leader said he would not be taking part because of "family reasons".
Channel 4 said they were notified at 2pm and the lineup has since been reshuffled.
The Prime Minister has slammed claims that leaving the EU would take back control for the UK as an "illusion".
On the last day of his Remain campaign at a rally in Birmingham, he said that leaving the European would not mean "taking control" but "losing control".
Asked if he thought his son, Boris Johnson, could become the next Prime Minister in the wake of a Brexit vote, Stanley Johnson replied he would be happy if anyone in his family is considered to have done a "useful job".
The chairman of Environmentalists For Europe who supports a remain vote, was campaigning in Bicester alongside Green Party MP Caroline Lucas, and former acting Labour leader Harriet Harman.
Mr Johnson also praised David Cameron's visit to Rainham Marshes Nature Reserve and statement on protecting the habitats of birds. However, Ms Lucas pointed out that should the UK vote to Leave the EU, Mr Cameron may no longer be Prime Minister.
Hopes of renegotiation of the UK's place in the European Union is an "absolute illusion", Boris Johnson has said.
The only way for the UK to have any control over its "borders, immigration arrangements, and democracy" is for it to leave the EU, the former London mayor said.
The Vote Leave campaigner also hit out at European Commission president Jean-Claude Juncker's claim that "out is out" if Britain votes for Brexit, and that the "Brussels bureaucrat" had "absolutely no intention of allowing this country [UK] to have any further change in its arrangements and obligations to the EU". This he said, left voters with no option but to "vote leave".
Mr Johnson made his comments on the final day of campaigning as he toured the country on behalf of Vote Leave.
"Tomorrow is not the day to cast a protest vote", Scottish Labour leader Kezia Dugdale MSP warned voters at a Labour In for Britain event.
Ms Dugdale told how she had "been here before", with regards to the future of a country in a referendum and urged voters to think about "what type of country do we want to be?"
She argued that as part of the European Union, the UK could work to tackle world issues such as the refugee crisis and climate change.
"The EU secures millions of jobs across UK, protects the rights of workers, and gives young people opportunities that were unthinkable to previous generations," the Scottish Labour leader said as she appeared at the event alongside Labour Leader Jeremy Corbyn, London Mayor Sadiq Khan, and First Minister of Wales Carwyn Jones AM at the event in Kings Cross, London.
London Mayor Sadiq Khan today warned voters that "out is out" and that there would be no going back from a vote to leave the European Union.
Speaking alongside Labour Leader Jeremy Corbyn, First Minister of Wales Carwyn Jones AM and Leader of Scottish Labour, Kezia Dugdale MSP, at a Labour In for Britain event in Kings Cross, London, Mr Khan said that the outcome of the referendum was a decision that would affect the UK "for decades to come".
Urging the electorate to vote for the UK to remain in the EU, the former Labour MP warned: "This is the fight of our lives".
Mr Khan added that working together as part of the EU, the UK would be "stronger, safer, and better off".
On the final day of campaigning before the EU referendum, Labour leader Jeremy Corbyn has urged the electorate to vote to remain in the European Union so that it can be reformed from within.
The Islington North MP said that while the EU "needs a lot of change and a lot of reform", he argued that it was best done from within.
He also urged the UK to use its presidency of the European Council in 2017 to outlaw zero hours contracts and improve workers' rights.
He also spoke about global tax avoidance and the need to work together to tackle pollution.
Labour Mayor of London, Sadiq Khan, First Minister of Wales Carwyn Jones AM and Leader of Scottish Labour, Kezia Dugdale MSP, also spoke at the Labour In for Britain event in Kings Cross, London.
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