The presenting duo filmed the message after hearing about Mila Sneddo's plea for people to stay at home to keep her safe.
Hosts Ant and Dec introduced a minute-long pause to the grand finale of the hit show.
The TV stars are set to return to our screens as a duo on Britain’s Got Talent.
The TV presenter, who stepped away from his on-screen roles last March, smiled alongside his presenting partner.
Dec married Ali Astall, his former manager, in 2015, and they announced they were to become first-time parents earlier this year.
The former Gogglebox star was crowned queen of the jungle in the 2016 series.
The duo’s programme Saturday Night Takeaway will not air next year while McPartlin takes his break.
TV presenter Ant McPartlin has been fined £86,000 for drink-driving alongside a 22-month ban.
The TV duo appeared together in the episode, which was recorded in January.
ITV said in a statement: "We send Ant all our love. And we know that Dec will do a brilliant job."