The TV presenter, who stepped away from his on-screen roles last March, smiled alongside his presenting partner.
The TV presenter has been out of the limelight for several months following his arrest for drink-driving earlier in 2018.
The TV star had confirmed earlier this year that he was splitting up with his estranged wife after 11 years of marriage.
Ant McPartlin is to take a break from television for a year, and will miss the next series of I'm A Celebrity and Saturday Night Takeaway.
TV presenter Ant McPartlin has been fined £86,000 for drink-driving alongside a 22-month ban.
The TV duo appeared together in the episode, which was recorded in January.
The presenter is seen laughing, dancing and at one point close to tears in the first episode of series 12 which was filmed back in January.
The Metropolitan Police have confirmed the charge following his arrest on Sunday. He will appear at Wimbledon Magistrates’ Court on April 4.
Britain's Got Talent host Ant McPartlin ends up at the sharp end of the show when an act drags him on stage for a daring sword trick.