Daniele De Rossi will call time on his 18-year career with Roma at the end of the Serie A season.
The 35-year-old midfielder made his Giallorossi debut under manager Fabio Capello in October 2001.
After making 615 appearances and scoring 63 goals, he has decided to leave the Stadio Olimpico following Roma's season-ending clash with Parma on May 25. He will not, however, be retiring from football.
"For 18 years, Daniele has been the beating heart of Roma's team," club president Jim Pallotta told asroma.com.
"From his debut in 2001 to taking on the responsibility of the captain's armband, he always represented the Roma fans on the pitch with pride as he established himself as one of the best midfielders in Europe.
"We will all shed tears when he pulls on the Giallorossi shirt for the last time against Parma, but we respect his desire to prolong his playing career - even if, at almost 36, it will be away from Rome.
"On behalf of everyone at Roma, I'd like to thank Daniele for his incredible dedication to this club and ensure him that our doors will remain open for him to return to the club in a new role whenever he wants."
De Rossi will address the media at Roma's Trigoria training centre at 1245 local time on Tuesday.
Roma have donated 150,000 euros (£133,000) to the family of injured Liverpool fan Sean Cox.
Irishman Cox was attacked by Roma supporters before the Champions League semi-final first leg at Anfield in April and is still recovering in hospital.
Footballing figures including Liverpool boss Jurgen Klopp and Everton defender Seamus Coleman have already made donations on Cox's family's GoFundMe page, and Roma have now followed suit.
A statement published on Thursday read: "Roma and club president Jim Pallotta have today announced that they will donate an initial sum of 150,000 euros to the family of Liverpool fan Sean Cox to help with his medical and rehabilitation costs.
"Roma will donate 100,000 euros through its Roma Cares foundation, while Pallotta has decided to make a personal donation of 50,000 euros.
"Today's announcement comes after club officials flew to Ireland on Friday to meet with Sean's wife Martina and representatives helping to manage the family's fundraising efforts.
"Having offered an initial financial donation, Roma have also agreed to work with the Cox family on further fundraising initiatives that will help cover ongoing costs for Sean over a multi-year period."
Roma also tweeted an invitation for Giallorossi fans to contribute, while players wore 'Forza Sean' (Come on Sean) T-shirts in training.
Marseille have announced the signing of Holland midfielder Kevin Strootman from Italian side Roma.
The Ligue 1 outfit have paid an initial 25million euros (£22.7million) for the 28-year-old, which could rise by an extra three million euros based on player and club performances. The length of his contract has not been disclosed.
Strootman is the latest player to leave the Italian capital in a big-money move following goalkeeper Alisson Becker's transfer to Liverpool and fellow midfielder Radja Nainggolan's departure to Serie A rivals Inter Milan earlier this summer.
The central midfielder made 44 appearances in all competitions last season as he helped the Giallorossi to third place in the table and the semi-finals of the Champions League, with a remarkable comeback to knock out Barcelona in the last eight.
Strootman played the full 90 minutes of Roma's season-opening 1-0 win at Torino last week but was left out of the matchday squad for Monday's 3-3 home draw with Atalanta as the deal moved closer to completion.
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Roma's sporting director Monchi has stressed selling Alisson "would not show a lack of ambition" as he confirmed the player was in Liverpool on Thursday.
Press Association Sport understands Liverpool have agreed to meet Roma's valuation of 75 million euros (approximately £67m) for Alisson, a figure which would make the 25-year-old Brazil international the most expensive goalkeeper in history.
On Wednesday, Italian website Forza Roma, which is affiliated to newspaper La Gazzetta Dello Sport, published a video which it said showed Alisson at Rome's Ciampino airport. It said he was preparing to board a private jet to Manchester and was on his way to Liverpool for a medical.
Speaking on Thursday at a press conference to present Roma's summer signing Justin Kluivert, Monchi was quoted on the Serie A club's official twitter feed as saying: "We haven't finalised anything yet (with Alisson).
"But it is true that the negotiations are advanced and he's over in Liverpool now."
Monchi, who took up his post at Roma in 2017, added: "When a very substantial offer comes in, you have to consider it.
"We weighed up the pros and cons and decided to speak to Liverpool.
"Selling Alisson would not show a lack of ambition. For me, ambition is about doing the right thing after thinking everything through.
"I came here to build a strong team, not just for one year but for many years to come - and I'm 100% sure that will be the case.
"I understand the fans' view. I want them to know we're working to build a stronger team.
"Some people never thought we'd be able to sign Kluivert. The same goes for (fellow summer signing Javier) Pastore.
"Perhaps today is tough for some fans, but we've brought in top players."
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Inter have confirmed they have completed the signing of midfielder Radja Nainggolan from Roma in a reported £21 million deal.
Nainggolan, who was left out of Belgium's squad for this summer's World Cup, has signed a four-year contract at San Siro.
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