Middlesbrough defender Ben Gibson has been called up to the senior England squad for the first time.
Chris Smalling has been forced out through injury, leading Ben Gibson to receive his first call-up.
Gareth Southgate was already facing a defensive headache for Sunday's World Cup qualifier against Lithuania as Gary Cahill, the skipper in Wednesday's 1-0 defeat in Germany, was suspended and Phil Jones withdrew through injury on the eve of the friendly.
The Manchester United defender sustained a toe injury in what appeared to be a training ground duel with team-mate Smalling, who on Friday left the squad with fitness problems of his own.
The centre-back has returned to Manchester for treatment on the unspecified issue, with Southgate turning to uncapped Middlesbrough defender Gibson.