The northbound Tyne Tunnel will be closed on Saturday and Sunday.
Operator TT2 Limited has said this is because of "essential maintenance" and has advised drivers to allow extra time for their journey.
Anyone driving towards will be directed to the southbound tunnel, which will operate as both north and southbound crossing, while the work takes place.
This move is expected to cause disruption.
The northbound tunnel will be closed from 6am on Saturday, 25 January until approximately 7pm on Sunday, 26 January.
TT2 has said extra staff will be available during the closure to help its customers.
A permit only lane will also be available for pre-pay customers.
Chris Ward, customer operations manager at TT2, said: