Pro-Russian separatists have declared independence for the south-eastern Ukrainian region of Lugansk, the Associated Press reports.
The move follows a referendum over the weekend that rebels say received the backing of over 90% of voters in Lugansk.
Earlier separatist leaders in Donetsk revealed they had asked the Kremlin to absord their region into the Russian Federation.
European Union foreign ministers added 13 people to their visa ban and asset freeze list over Ukraine's crisis.
Well that’s the voting over, and no surprises so far. A ‘Yes’ vote claimed to be 89% in Donetsk, and suddenly Ukraine is no more.
Nearly 90 percent of voters in eastern Ukrainian region voted for self-rule, the head of the separatist election commission said.