Forty-five migrants were taken by ferry from Lesbos to Turkey as Greek authorities restarted returns under an EU deal to stem the flow of migrants and refugees to Europe.
The ferry left the port of Mytilene on Friday morning, with a second boat expected to leave later, state television reported.
The first group of 202 migrants to be returned, most of them from Pakistan and Afghanistan, were taken back to Turkey on Monday.
Turkish President Tayyip Erdogan criticised European countries for not letting migrants into their territories by raising razor wire fences.
Speaking to the general assembly for humanitarian organisation Red Crescent in Ankara, he said that European countries "turned back Syrians" while the Turkish government had rescued 100,000 migrants from the Aegean Sea.
Greece has begun returning migrants to Turkey, home to 2.7 million migrants, as part of an EU agreement for financial and political concessions.
The Democratic presidential candidate may also have shown his cards on his choice of running mate.
The US president also shared a post on Twitter accusing Dr Anthony Fauci of misleading the public over hydroxychloroquine.
Fears over an impending second wave of coronavirus dominates Wednesday’s front pages.