Boris Johnson said Britain would still have access to the single market if it left the EU.
This claim was met by further heckling from the remain side, with Angela Eagle saying he was "making it up".
Mr Johnson said the remain side were resorting to "Project Fear" and highlighted a comment by Nicola Sturgeon that the remain campaign was "miserable, negative and fear-based".
Campaigners for remaining in and leaving the EU have clashed in a fiery debate on ITV.
On a night of fierce debate between Remain and Leave campaigners during the ITV referendum debate, which of the claims actually stacked up?
Analysis of ITV's EU referendum debate.