Gisela Stuart said the EU referendum was the "last chance to take back control".
Ms Stuart, of leave, said Britain did not have to "choose between trade and democracy" and that a vote to remain was "dangerous".
"If you would not vote to join now, then do not vote to stay," she said in her closing speech.
Campaigners for remaining in and leaving the EU have clashed in a fiery debate on ITV.
On a night of fierce debate between Remain and Leave campaigners during the ITV referendum debate, which of the claims actually stacked up?
Analysis of ITV's EU referendum debate.