Ben Chilwell and Demarai Gray received their first England call-ups as Luke Shaw prepares to return to Manchester United after sustaining concussion against Spain.
Gareth Southgate's men started their first ever UEFA Nations League campaign with a 2-1 defeat to La Roja at Wembley on Saturday evening.
England are back in action against Switzerland in a friendly at the King Power Stadium on Tuesday, when Chilwell and Gray could make their debuts at their home ground.
The Leicester duo move up from England Under-21s as left-back Shaw prepares to head back to his club.
The 23-year-old's England return came to a shuddering halt on Saturday, when he was hurt in a collision with Dani Carvajal early in the second half.
A statement from the Football Association read: "Luke Shaw remained at St. George's Park today for further rest and monitoring having sustained concussion against Spain yesterday. He will return to his club tomorrow."