More than a third of LGBT employees in Wales hid their sexuality in their workplace in the last year in fear of discrimination.
New research from Stonewall Cymru revealed exclusion and discrimination in workplaces.
Stonewall Cymru say workplace bullying continues to be a serious problem for LGBT people.
This includes being the target of derogatory remarks, experiencing bullying and abuse, and being outed without consent.
The situation for trans people, disabled LGBT people and LGBT people in lower income households is particularly bad, with all groups facing higher levels of abuse and exclusion in the workplace.
Stonewall Cymru is calling for employers to develop zero-tolerance policies on homophobic, biphobic and transphobic discrimination and harassment, alongside communicating clear routes to report anti-LGBT bullying.
The charity is also calling for employers to take an active role in supporting trans staff by running awareness sessions for all employees on trans inclusion and developing policies for staff who transition at work.
Andrew White, Director of Stonewall Cymru, says while a growing number of employers are showing dedication to inclusiveness, more needs to be done:
based on YouGov research of 825 lesbian, gay, bi and trans employees across Wales,