Twenty-five GP surgeries have closed in Wales between October 2015 and March 2019, according to the British Medical Association.
Many more surgeries are said to be "at risk" of closure.
The most recent to close its doors was Bromfield Medical Centre in Mold, known by patients as Dr Tom's, which shut suddenly on Thursday with "immediate effect".
Sheila Lloyd and her 61-year-old mother Kathleen Roscoe have been patients there since it first opened in 1990. The pair from Deeside said they are "shocked and upset" and unsure of what to do next.