The only direct air route linking Wales and Germany, a weekly flight between Cardiff and Dusseldorf, will not operate next year unless a new airline takes over the service.
Germanwings, which is owned by the Lufthansa Group, launched the low cost flights in April, as a seasonal service which ran until last month. The German route was seen as an important part of a strategy to bring more tourists to Wales. Visit Wales, the tourism arm of airport owner the Welsh Government, was closely involved in securing the Dusseldorf flights.
Cardiff Airport is still trying to renew the service for 2015.
However, Germanwings confirmed to ITV News Cymru Wales that the airline has pulled out of Cardiff.
The Welsh Government has been asked to comment. The Conservative opposition in the Assembly see this latest development as a further sign of trouble for the Welsh Government's running of Cardiff Airport, which it bought for £52 million.